Table 2.
Sperm quality variables (LSMeans ± SE) and p values in fresh ejaculates of selected semen donors for cryobanking in Bionda Piemontese (BP), Bianca di Saluzzo (BS) and Pepoi (PP) Italian chicken breeds.
| Sperm Variables 1 |
Breeds | p | ||
|---|---|---|---|---|
| BP | BS | PP | ||
| VOL (mL) | 0.43 ± 0.03 | 0.35 ± 0.03 | 0.41 ± 0.02 | ns |
| CONC (×109/mL) | 3.11 ± 0.17 a | 3.67 ± 0.16 b | 4.44 ± 0.11 c | 0.0001 |
| TSO (×109) | 1.34 ± 0.15 a | 1.35 ± 0.15 a | 1.89 ± 0.10 b | 0.0012 |
| SMI (%) | 91.59 ± 1.14 a | 91.00 ± 1.09 a | 95.09 ± 0.77 b | 0.0029 |
| TMS (%) | 89.52 ± 1.65 | 90.71 ± 1.57 | 92.54 ± 1.10 | ns |
| PMS (%) | 25.78 ± 1.38 a | 24.58 ± 1.32 a | 29.90 ± 0.92 b | 0.0018 |
| VCL (μm/s) | 77.55 ± 3.19 a | 76.59 ± 3.05 a | 67.36 ± 2.14 b | 00.79 |
| VSL (μm/s) | 30.02 ± 1.27 | 28.43 ± 1.21 | 29.30 ± 0.85 | ns |
| VAP (μm/s) | 48.43 ± 2.01 | 47.05 ± 1.92 | 44.68 ± 1.35 | ns |
| LIN (%) | 39.49 ± 0.82 a | 37.37 ± 0.78 a | 43.64 ± 0.55 b | 0.0001 |
| STR (%) | 62.44 ± 0.72 a | 60.57 ± 0.69 a | 65.65 ± 0.48 b | 0.0001 |
| WOB (%) | 62.90 ± 0.64 a | 61.42 ± 0.61 a | 66.23 ± 0.43 b | 0.0001 |
| ALH (μm) | 3.89 ± 0.08 a | 3.92 ± 0.08 a | 3.27 ± 0.05 b | 0.0001 |
| BCF (Hz) | 6.95 ± 0.10 a | 6.91 ± 0.09 a | 7.74 ± 0.06 b | 0.0001 |
a,b. Means within a row with different letters are significantly different. 1 VOL, sperm volume; CONC, sperm concentration; TSO (VOL*CONC), total sperm output; SMI, sperm membrane integrity; TMS, total motile sperm; PMS, progressive motile sperm; VCL, curvilinear velocity; VSL, straight-line velocity; VAP, average path velocity; LIN (VSL/VCL × 100), linearity; STR (VSL/VAP × 100), straightness; WOB (VAP/VCL × 100), wobble; ALH, amplitude of lateral head displacement; BCF, beat cross frequency; ns = not significant.