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. 2024 Mar 27;11(4):148. doi: 10.3390/vetsci11040148

Table 3.

Sperm quality variables (LSMeans ± SE) and P of the breed factor in frozen/thawed ejaculates of selected donor males for cryobanking in Bionda Piemontese (BP), Bianca di Saluzzo (BS) and Pepoi (PP) Italian chicken breeds.

Sperm
Variables 1
Breeds p
BP BS PP
SMI (%) 38.00 ± 3.88 31.60 ± 3.47 31.09 ± 2.39 ns
TMS (%) 31.22 ± 4.53 29.22 ± 4.05 28.28 ± 2.79 ns
PMS (%) 2.91 ± 0.87 3.02 ± 0.78 3.53 ± 0.53 ns
VCL (μm/s) 36.76 ± 1.71 36.77 ± 1.53 36.62 ± 1.06 ns
VSL (μm/s) 11.71 ± 1.24 11.57 ± 1.11 11.98 ± 0.77 ns
VAP (μm/s) 20.21 ± 1.58 20.16 ± 1.41 20.35 ± 0.97 ns
LIN (%) 31.75 ± 2.31 31.02 ± 2.07 31.89 ± 1.43 ns
STR (%) 57.62 ± 2.07 56.75 ± 1.85 57.31 ± 1.27 ns
WOB (%) 54.92 ± 2.29 54.29 ± 2.05 54.78 ± 1.41 ns
ALH (μm) 3.21 ± 0.15 a 2.85 ± 0.13 ab 2.52 ± 0.09 b 0.0014
BCF (Hz) 6.52 ± 0.61 6.18 ± 0.55 6.70 ± 0.38 ns

a,b. Means within a row with different letters are significantly different. 1 VOL, sperm volume; CONC, sperm concentration; TSO (VOL*CONC), total sperm output; SMI, sperm membrane integrity; TMS, total motile sperm; PMS, progressive motile sperm; VCL, curvilinear velocity; VSL, straight-line velocity; VAP, average path velocity; LIN (VSL/VCL × 100), linearity; STR (VSL/VAP × 100), straightness; WOB (VAP/VCL × 100), wobble; ALH, amplitude of lateral head displacement; BCF, beat cross frequency. ns = not significant.