Figure 7.

SARS-CoV-1 VLP Characterisation and Neutralising Antibody Responses. (A) Expression of S, M and E SARS-CoV-1 proteins in insect cells infected with a triple recombinant baculovirus, analysed by SDS-PAGE followed by Coomassie blue staining. (B) EM of immunogold-labelled SARS-CoV-1 VLPs probed with anti-S monoclonal antibody (adapted from [39]). (C) IC90 neutralising antibody titre against SARS-CoV-1 pseudotyped lentivirus, using sera from 3 mice immunised with SARS-CoV-1 VLPs, 1 mouse immunised with Rotavirus VLP and one serum obtained from a SARS-CoV-1 convalescent patient (adapted from [40]).