Figure 5.

Treatment of primary macrophages with cART inhibits autophagosome fusion with the lysosome. Primary macrophages were infected with HIV-SF162 at an MOI of 1 and treated with antiretrovirals every 48 h. Autophagy was assessed using an RFP-GFP-LC3B tandem autophagy sensor. The experiments were performed in two independent donors, Donor 1 (A,B) and Donor 2 (C,D) +/− HIV-1 infection. Autophagy was measured at 24, 48, and 96 h post-treatment, and data points represent an average of 12 technical replicates.