TABLE 3.
Detection of Ba-L and NL4-3 proviruses in sorted thymic cells
| Cohort | Day post-inoculation | Virus | Mouse no. | No. of proviruses/ 1,000 autofluorescent cellsa | No. of proviruses/1,000 CD4+ CD8+ thymocytesa | Ratiob |
|---|---|---|---|---|---|---|
| 1 | 19 | Ba-L | 12 | 5 | <1 | >5 |
| 13 | 5 | <1 | >5 | |||
| 14 | 5 | <1 | >5 | |||
| 15 | 25 | <1 | >25 | |||
| 2 | 14 | Ba-L | 25 | 5 | <1 | >5 |
| 26 | 1 | <1 | >1 | |||
| 27 | 25 | <1 | >25 | |||
| 28 | 1 | <1 | >1 | |||
| NL4-3 | 15 | 5 | 25 | 0.2 | ||
| 16 | 25 | 100 | 0.25 | |||
| 5 | 14 | Ba-L | 17 | 25 | <1 | >25 |
| 21 | Ba-L | 18 | 5 | 5 | 1 | |
| 20 | 5 | 5 | 1 | |||
| 21 | 25 | 5 | 5 | |||
| NL4-3 | 12 | 5 | 25 | 0.25 | ||
| 13 | 25 | 125 | 0.25 | |||
| 16 | 25 | 25 | 1 |
a
Calculated from the highest dilution of lysed cells which yields a detectable PCR product (for example, HIV-1 DNA could be detected by PCR in 40 but not 8 autofluorescent cells from mouse 17; thus, 40 cells must contain at least one provirus, and 1,000 cells must contain at least 25 proviruses).
b
Ratio of the frequency of provirus in autofluorescent cells over the frequency of provirus in CD4+ CD8+ thymocytes.