Effects of permeating and nonpermeating solutes on monounsaturated and saturated fatty acids. (A) Percentage of 16:1 and 18:1 fatty acids; (B) percentage of total monounsaturates; (C) saturated:unsaturated fatty acid ratio. Open symbols, 18:1 fatty acids; closed symbols, 16:1 fatty acids (A); ●, PEG 8000; ■, PEG 200, ▴, NaCl (B and C). Values are means ± SE of three to five replications. LSDs for the percentage of 16:1 fatty acids (A) are 2.6, 1.4, and 1.7 for PEG 8000, PEG 200, and NaCl treatments, respectively. LSDs for the percentage of 18:1 fatty acids (A) are 2.7, 1.9, and 2.2 for PEG 8000, PEG 200, and NaCl treatments, respectively. LSDs for the percentage of unsaturated fatty acids (B) are 1.3, 1.7, and 1.7 for PEG 8000, PEG 200, and NaCl treatments, respectively. LSDs for the saturated:unsaturated fatty acids ratio (C) are 0.03, 0.03, and 0.02 for PEG 8000, PEG 200, and NaCl treatments, respectively.