TABLE 2.
Effect of hyperosmotic shock on cis-to-trans isomerizationa
| Treatment | Fatty acid content (%)b
|
||||||
|---|---|---|---|---|---|---|---|
| 16:1ω7c | 16:1ω7t | 18:1ω7c | 18:1ω7t | Total cis | Total trans | Total trans:cis ratio | |
| None | 29.1 ± 3.6 A | 5.7 ± 1.9 A | 22.3 ± 0.2 A | 0 ± 0 A | 51.3 ± 3.8 A | 5.7 ± 1.9 A | 0.10 ± 0.04 A |
| NaCl | 23.4 ± 1.1 B | 10.7 ± 1.5 B | 22.6 ± 0.4 A | 0 ± 0 A | 46.0 ± 1.5 B | 10.7 ± 1.5 B | 0.24 ± 0.03 B |
| PEG 8000 | 16.0 ± 0.8 C | 17.3 ± 0.8 C | 20.0 ± 1.1 B | 2.6 ± 1.6 B | 36.0 ± 2.7 C | 19.9 ± 2.4 C | 0.55 ± 0.09 C |
a
Mid-exponential-phase cells were grown in a basal medium and suddenly exposed to a −1.5-MPa reduction in solution water potential by the addition of the permeating solute NaCl or the nonpermeating solute PEG 8000. Fatty acids were extracted from cells within 15 min after the addition of NaCl or PEG 8000. Values are the means ± SE of three replications.
b
Values followed by the same letter in a column are not statistically different, based on Fisher's LSD (P ≤ 0.05).