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CD spectra of different mutant forms of the 14-kDa protein. (A) Far-UV CD spectrum of the 14K-A mutant form of the 14-kDa protein. (B) pH dependence of the ellipticity at 222 nm for the 14K-A mutant. (C) Far-UV CD spectrum of a peptide corresponding to the predicted Leu zipper region at the C terminus of the 14-kDa protein, at two different concentrations (20 and 500 μM), to show the concentration dependence of its ellipticity. Open circles, 20 μM; solid circles, 500 μM.
