Table 3.

Mutagenesis assay without metabolic activation to assess cytotoxicity.

Mutagenesis Assay without Metabolic Activation
Strain	Test Item	Dose Level per Plate	Mean Revertants per Plate	Standard Deviation	Ratio Treated/Solvent	Individual Revertant Colony Counts
TA98	%10 Sucrose	61.3 µL	22	4.4	-	20,27,19
	AVD-104 31		21	5	1	21,26,16
		93	15.7	4.7	0.7	12,21,14
		278	19.7	0.6	0.9	20,19,20
		833	8.3	2.5	0.4	6,11,8
		2500	15.3	2.5	0.7	13,18,15
	2NF	2.5	603.3	78.2	27.4	549,568,693
TA100	%10 Sucrose	61.3 µL	119	5	-	114,119,124
	AVD-104	31	104.7	16.8	0.9	94,96,124
		93	131.3	13.3	1.1	139,139,116
		278	127.7	11.1	1.1	138,116,129
		833	129.7	13.8	1.1	140,114,135
		2500	105.3	17.6	0.9	91,125,100
	SA	1	619.3	152.2	5.2	776,472,610
TA1535	%10 Sucrose	61.3 µL	11.7	0.6	-	12,12,11
	AVD-104	31	9.7	3.8	0.8	8,7,14
		93	9.3	5.1	0.8	5,8,15
		278	12	1	1	11,12,13
		833	7.7	1.5	0.7	9,6,8
		2500	9.7	1.2	0.8	9,9,11
	SA	1	639.7	17.6	54.8	656,621,642
TA1537	%10 Sucrose	61.3 µL	5.7	2.1	-	5,8,4
	AVD-104	31	3.3	2.1	0.6	1,5,4
		93	7	1	1.2	6,8,7
		278	10	1.7	1.8	12,9,9
		833	6.3	1.2	1.1	7,5,7
		2500	7	1	1.2	8,7,6
	ICR	0.5	118.3	6.7	20.9	126,115,114
WP2uvrA	%10 Sucrose	61.3 µL	29.3	9.5	-	40,22,26
	AVD-104	31	30.7	2.5	1	31,33,28
		93	30.7	11.2	1	35,18,39
		278	33	9	1.1	24,33,42
		833	30	10.1	1	21,28,41
		2500	42.3	14.8	1.4	26,55,46
	NQNO	2	907.3	85.6	30.9	929,980,813

Vehicle & Positive Controls. %10 sucrose, 2NF: 2-Nitrofluorene, ICR: ICR-191 Acridine, NQNO: 4-nitroquinoline-N-Oxide, SA: Sodium azide.