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Relative expression level of genes involved in sterol biosynthesis and thiol metabolism treated with M. nervosa EO on L. major promastigotes. Negative control: untreated parasites, AmpB: amphotericin B. The relative expression of CYP51, ODC, SPS, TRYS, TR, CTP, and MTP genes was quantified by qPCR using 18SrRNA housekeeping genes as normalization control.
