FIG. 7.

Mutations at serine 118 do not significantly influence viral replication. Primary duck hepatocytes were infected with wild-type (wt) DHBV or variant viruses (carrying L-S118A or L-S118D) produced from LMH cells. The viral DNA contents in tissue culture supernatants collected at the indicated time intervals were determined by dot blot analysis relative to a DHBV DNA standard by using a PhosphorImager. Each point is the average of values from two wells.