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Kinetic parameters for purified β-galactosidase determined at different temperaturesa
| Temp (°C) | Vmax (μmol of o-nitrophenol produced/min/mg of protein) | kcat (s−1) | Km (mM ONPG) | Catalytic efficiency (M−1 s−1) |
|---|---|---|---|---|
| 1.9 | 63 | 81.8 | 7.4 | 11,000 |
| 10 | 80 | 104 | 4.5 | 23,000 |
| 20 | 223 | 288 | 5.4 | 54,000 |
| 30 | 392 | 507 | 5.0 | 101,000 |
| 39 | 467 | 603 | 4.9 | 123,000 |
To determine the values, we assumed that there is one active site per subunit in a dimeric enzyme.
