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Calibration curves of SYBR Green-based qPCR primers Spike del 143-145, N del 31-33, Spike control and TaqPath kit. Serially 10-fold diluted RNA containing the mutations S Δ143-145 and N Δ31-33 were amplified and analyzed. (a) The threshold cycle (Ct) mean values were plotted against the Log starting quantity (ng/μl). slope, R2 and Efficiency (E) were determined. Each dilution was assayed in triplicate.
