Table 1.
Target gene | Primer name | Primer sequence (5’-3’) |
---|---|---|
N | N WT 31-33 forward | GTAACCAGAATGGAGAACGCA |
N | N del 31-33 forward | ACTGGCAGTAACCAGAATGGTG |
N | N reverse | CGGTAGTAGCCAATTTGGTCATC |
S | Spike WT 143-145 forward | ATGATCCATTTTTGGGTGTTTATT |
S | Spike del 143-145 forward | GTAATGATCCATTTTTGGACCA |
S | Spike reverse | ACACAAATTCCCTAAGATTTTTGA |
S | Spike control forward | CAACAAAAGTTGGATGGAAAGTG |
S | Spike control reverse | GATCACGCACTAAATTAATAGGC |
Deleted nucleotides are in bold.