Fig. 9.
p190RhoGAP regulates substratum rigidity–induced endothelial differentiation through a RhoA-independent mechanism. (A to D) In scrKD cells cultured on MRS for 3 days (A) and 10 days. (B), GATA2 changes from a mostly perinuclear localization to a mostly nuclear localization (arrows). In p190KD cells cultured on MRS for 3 days (C) and 10 days (D), GATA changes from a nuclear and cytoplasmic localization to a predominantly nuclear localization by day 10 (arrows). (E) Analysis of perinuclear, and nuclear GATA2 localization in wild-type CDCs and p190KD CDCs cultured on glass and MRS for 3, 7, and 10 days. (F) Schematic showing the putative role of the rigidity-induced decrease in p190RhoGAP abundance in promoting cell proliferation, endothelial differentiation, and morphogenesis through regulation of RhoA activation and abundance and subcellular localization of GATA2. Scale bars, 20 μm (A to D). *P < 0.05; **P < 0.01. Error bars, SEM.