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. 2024 Apr 20;14(8):e4972. doi: 10.21769/BioProtoc.4972

Table 1. Examples with respect to calculation of T-cell transfection volume.

Parameters Example 1 Example 2 Example 3
T-cells per TCR transfection 2.5 × 106 5 × 106 7 × 106
TCR mRNA 2.5 µg 5 µg 7 µg
T-cell transfection volume 125 µL 250 µL 350 µL