Figure 5.

Changes in Ca²⁺ accumulation in salt glands of diploid (2x) and tetraploid (4x) Plumbago auriculata induced by NaCl stress and effects of LaCl₃, Na₃VO₄, and DPI on Ca²⁺ accumulation under NaCl stress. (A) Representative graphs showing changes in Ca²⁺ accumulation in (top) diploid and (bottom) tetraploid salt glands before and after salt stress, as well as effects under LaCl₃, Na₃VO₄, and DPI treatments. (B) Fluorescence intensity values from (A) measured by Image J software. For each treatment, nine salt glands from six individual plants were observed and quantified. In (B), different capital letters indicate significant differences (P < 0.05) between different treatments of the same ploidy level, whereas different lowercase letters indicate significant differences (P < 0.05) between ploidy levels of the same treatment.