FIG. 4.

Requirement for lef-12 in transient late gene expression assays. SF-21 cells (A and B) or TN-368 cells (C and D) were transfected with the AcMNPV genomic library (A and C) or the HSEpiHis lef library (B and D). Library clones supplied or omitted from the complete libraries are indicated below each graph. In panel C, plasmid clones pAcIAP-PstI/NsiI and pBS-RI-M were added to supply lef-6 and lef-8, respectively, and plasmid pFspAfl (51) was added to supply both 39k and lef-11. Plasmids supplying wild-type p47 or lef-12 or corresponding frameshift mutations are shown below the lanes. CAT activities are reported relative to those of the full libraries; data represent the mean of at least three independent experiments, and bars represent the standard error.