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. 2024 Jan 7;57(5):e13593. doi: 10.1111/cpr.13593

FIGURE 1.

FIGURE 1

Isolation and identification of cardiopulmonary progenitors (CPPs). (A) Regions for isolating CPPs from 9.5‐day embryos. Scale bar, 2 mm. (B) Schematic diagram of the workflow for CPPs culture in vitro with representative photos. Scale bar, 1 mm. (C) Bar plots showing the cell numbers of CPPs cultured from passage number 0 (P0) to 6 (P6) in vitro. Data are shown as mean ± SEM; n = 3 biological replicates per group; ***p < 0.001 (t‐test). (D) Identification of CPPs markers (Isl1, Wnt2, and Gli1) by immunofluorescence. Upper images: scale bar, 100 μm; lower images: scale bar, 50 μm. (E) Flow cytometric analysis of CPPs for expression of the CPPs markers Isl1, Wnt2, and Gli1. (F) Immunofluorescence staining of CPPs for the expression of the stemness marker Mesp1. Upper images: scale bar, 200 μm; lower images: scale bar, 100 μm. (G) Identification of mRNA levels of stemness markers, such as Mesp1, Ssea1, Pdgfra, Mef2c, Kdr, and Nkx2.5 in CPPs. Data are shown as the mean ± SEM; n = 3 biological replicates per group; ***p < 0.001 (t‐test).