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Representative images and quantification of 3D spheroids derived from RMS cell lines (a) RD and (b) RH-30 treated with 1/2IC50, IC50, and 2× IC50 concentrations of the trinuclear ruthenium(II) arene complex 1 or cisplatin for 6 days. The area of the spheroids was determined using ImageJ. The graphs represent the mean normalized spheroid size ± SEM for each treatment condition, where *p ≤ 0.05 and **p ≤ 0.01, and thus statistically significant.
