Table 2.

Troubleshooting table.

Step	Problem	Possible reasons	Solutions
14	No nuclei pellet	Spin conditions	Extend spin time or speed
38	Loss of nucleosomal banding	Chromatin integrity disrupted during cell collection	Try alternative methods of cell dissociation during collection to maintain high viability
		Over-tagmentation	Scale down reaction size relative to cell number or increase the starting number of cells
			Decrease transposition incubation time
	Accumulation of large size fragments	Under-tagmentation	Reduce cell numbers or scale up reaction size relative to cell number
			Increase transposition incubation time
69	Low yield	Insufficient lysis	Additional pipetting following addition of lysis buffer
		Inaccurate cell count	Repeat cell counting, confirming single cell suspension has been achieved and viability is high (>80%)