Component	Amount (μl)	Final concentration
10 μM Tn5mC-Repl01 oligo (Table 1)	2	1 μM
10x Ampligase buffer	2	1x
50 mM NAD+	0.5	1.25 mM
10 mM dNTPs (2.5 mM of each of the 4 dNTPs)	2	1 mM
Total	6.5