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. Author manuscript; available in PMC: 2024 Apr 29.
Published in final edited form as: Nat Protoc. 2021 Oct 18;16(12):5377–5397. doi: 10.1038/s41596-021-00608-z
Component Amount (μl) Final concentration
NEBNext Ultra II Q5 Master Mix 10 1x
10 μM Reverse Primer (N5 index; Table 1) 1 0.5 μM
10 μM Forward Primer (N7 index; Table 1) 1 0.5 μM
Purified gap-repaired DNA fragments (step 34) 2
Nuclease-free water 6
Total 20