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. 2024 Apr 29;15:125. doi: 10.1186/s13287-024-03738-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Transcriptomic analysis of busulfan-treated small intestine organoids. A Busulfan-treated organoids or control organoids were co-cultured with CellTraceTM violet dye-labelled MSCs for 48 h and single cell small intestine organoid were isolated from these co-cultures by FACS sorting. B DE analysis between control and busulfan-treated small intestine organoids (red dots indicate significant genes; p value < 0.05) and the accompanying upregulated (C) and downregulated (D) pathways. E Heat map of the differentially expressed genes involved in the EMT, proliferation, and apoptosis pathway in control organoids and busulfan-treated organoids is shown (N = 3)