TABLE 3.
Surface water results for detection of enteroviruses with the BGMK cell line
| Site | Concentrate
|
ICC with BGMK cells
|
ICR TCVA-MPN/100 litersg | ||||
|---|---|---|---|---|---|---|---|
| RT-PCRa | Nested PCR
|
RT-PCRd | Nested PCR
|
||||
| 101b | 1:5c | 101e | 1:5f | ||||
| 1 | −h | − | NA | + | + | NA | |
| 2 | − | + | + | − | + | + | |
| 3 | − | + | + | − | + | + | 2.11 (0.08–9.41)i |
| 4 | − | − | NA | − | − | NA | |
| 5 | − | + | + | − | + | + | |
| 6 | − | − | NA | − | − | NA | |
| 7 | − | − | NA | − | − | NA | |
| 8 | − | + | + | + | + | + | 1.03 (0.18–3.48) |
| 9 | − | − | NA | − | − | NA | 2.35 (0.18–7.43) |
| 10 | − | + | − | − | + | + | |
| 11 | − | + | − | − | + | + | |
| 12 | I | I | I | − | + | NA | |
| 13 | − | + | + | − | + | + | |
| 14 | − | + | − | + | + | + | |
| 15 | I | I | I | − | − | NA | |
| 16 | I | I | I | − | − | NA | |
| 17 | − | − | NA | − | − | NA | |
| 18 | − | + | + | − | + | + | |
| 19 | − | + | + | − | + | + | |
| 20 | − | + | + | − | + | + | 1.03 (0.19–3.47) |
| 21 | − | − | NA | − | − | NA | |
| 22 | − | − | NA | + | + | NA | |
| 23 | − | + | + | + | + | + | 3.23 (0.18–7.39) |
| 24 | I | I | I | − | − | NA | |
| 25 | I | I | I | − | − | NA | |
| 26 | − | − | NA | − | − | NA | |
| 27 | − | − | NA | − | − | NA | |
| 28 | − | + | + | + | + | + | |
| 29 | − | − | NA | − | + | NA | |
a
None of the 29 samples tested was positive.
b
Thirteen of 29 samples (44.8%) were positive.
c
Ten samples were positive.
d
Six of 29 samples (20.6%) were positive.
e
Seventeen of 29 samples (58.6%) were positive.
f
Fourteen samples were positive.
g
Five of 29 samples were positive.
h
−, negative; +, positive; NA, not applicable; I, inhibitory for the RT-PCR–nested PCR as determined by seeded experiments.
i
The values in parentheses are confidence intervals.