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. 2021 Jul 2;7(27):eabf8631. doi: 10.1126/sciadv.abf8631

Fig. 1. Germline Ssbp1 knockout leads to embryonic lethality, whereas knockout in the heart and skeletal muscle leads to cardiomyopathy.

Fig. 1

(A) Targeting strategy for disruption of the Ssbp1 gene. Blue arrowhead, loxp sequence; green arrowhead, frt sequence. (B) Three polymerase chain reaction (PCR) primers designed for detecting Ssbp1 exon 3. (C) Genotyping of Ssbp1+/+, Ssbp1+/−, and Ssbp1−/− tissues by indicated primers. (D) Morphology of Ssbp1+/+ and Ssbp1−/− embryos at E8.5. Scale bar, 0.5 mm. (E) Relative mtDNA levels in Ssbp1+/+, Ssbp1+/−, and Ssbp1−/− embryos assessed with real-time quantitative PCR (qPCR). Data are represented as means ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001. (F) Survival curve of Ssbp1 conditional knockout mice (L/L, cre) and controls (L/L). (G) Heart-to-body weight ratio of L/L, cre and L/L. (H and I) Hematoxylin and eosin staining and COX/SDH staining of heart tissues from 17-week-old L/L, cre and L/L. Scale bars, 1 mm (H) and 100 μm (I).