Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Feb 15;149(18):1419–1434. doi: 10.1161/CIRCULATIONAHA.123.065597

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 The Authors.

Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

PMC Copyright notice

Figure 6. — Incubation with of Tet2-deficient bone marrow–derived macrophages alters cardiomyocyte calcium handling. A, Human pluripotent stem cells differentiated to atrial cardiomyocytes (hPSC-aCM) were coincubated with Tet2-deficient (Tet2KO) bone marrow–derived macrophages (BMDM) or cytokines, IL-1β and IL-6. Calcium studies were performed using the calcium sensitive dye Fluo-3 AM. B, After 1 minute pacing at 1 Hz, fast perfusion of 10 mM caffeine leads to calcium emptying into the cytosol from SR. C, Tet2KO BMDM-treated hPSC-aCM showed reduced total SR Ca2⁺ release. △F/F0 is the change in fluorescence intensity over baseline. D, 1 Hz paced calcium transient amplitude (measured with Fluo-3 AM) is reduced in the hPSC-aCM incubated with Tet2KO BMDM compared with WT BMDM, with E, an increase in calcium transient time to peak (TTP). F, hPSC-aCM cardiomyocytes stimulated in culture with cytokines IL-1β 10 ng/mL or IL-6 10 ng/mL showed decreased SR calcium release (measured with Fluo-4 NW). Statistical tests: 2-tailed Student t test for C through E; 1-way ANOVA with Tukey multiple comparisons for F. IL-1β indicates interleukin 1β; IL-6, interleukin 6; SR, sarcoplasmic reticulum; TET2, tet methylcystosine dioxygenase 2; and WT, wild-type.