Fig. 2 |. Identification of IL-27 not IL-35 as a potential suppressor molecule specifically produced by intestinal T_reg cells under autoimmune inflammation.

a–c, Violin plots of Il27 (a), Ebi3 (b) and Il12a (c) in T_conv and T_reg cells in different tissues from control PBS- or DT-treated Foxp3^DTR mice by RNA-seq analysis. d–f, The qPCR analyses for the expression of Il27 (d), Ebi3 (e) and Il12a (f) in T_conv and T_reg cells in different tissues from control PBS- or DT-treated mice. Each symbol represents an individual mouse (n = 5). g,h, ELISA of the production of IL-27 (g) or IL-35 (h) by T_conv and T_reg cells in different tissues from control PBS- or DT-treated mice. i,j, The qPCR analyses for the expressions of Il27 (i) and Ebi3 (j) in T_conv and T_reg cells in SI from control PBS- or aCD3⁻-treated SPF or GF mice. Each symbol represents a FACS-isolated cell sample pooled from two to three mice (n = 4). k, ELISA analyses of the production of IL-27 by T_conv and T_reg cells in SI LP from control PBS- or aCD3 monoclonal antibody-treated SPF and GF mice. Each symbol represents a FACS-isolated cell sample pooled from two to three mice (n = 2 for SPF and 3 for GF). The dotted line represents the minimum detection limit of the indicated cytokine. The data are presented as mean values ± s.d. In d, *P = 0.0164 (up), 0.0187 (middle) and 0.0173 (bottom). In e, ***P = 0.0004 (up), 0.0007 (middle) and 0.0008 (bottom). In g, ****P < 0.0001 (top), 0.0003 (middle) and 0.0003 (bottom). In i, *P = 0.0351 (left) and 0.0394 (right). In j, *P = 0.0467 (left) and 0.0193 (right). In k, *P = 0.0413 (left) and 0.0207 (right). Statistical significance was determined using a two-tailed, unpaired Student’s t-test.