Fig. 7 |. Single-cell analyses identify a putative IL-27-producing T_reg cell subset in the inflammatory intestinal tissue.

a, UMAP plots of intestinal T_reg cell clusters, colored by cluster identity. b, Heatmap of the top ten DEGs between each intestinal T_reg cell cluster. c, Percentage of cells within each intestinal T_reg cell cluster from mice with or without C. rodentium infection. d–g, Violin plots of Il27 (d), Ebi3 (e), Cd83 (f) and Tcf7 (g) in different intestinal T_reg cell clusters from C. rodentium-infected mice. h, FACS analysis of intestinal CD4⁺Foxp3⁺ T_reg cell clusters based on the expression of CD83 and TCF1. i, Representative FACS profiles with gating strategy for isolating different intestinal T_reg cell subsets based on the expression of CD83 and CD62L. j–l, The qPCR analyses for the expressions of Il27 (j), Ebi3 (k) and Il10 (l) in different T_reg cell subsets in spleen (Spl) or LI LP from C. rodentium-infected mice. Each symbol represents a FACS-isolated cell sample pooled from three mice (n = 5). Data are presented as mean values ± s.d. In j, *P = 0.0441 (up) and 0.0186 (bottom left), and NS = 0.0806 (bottom right). In k, ***P = 0.0005 (up), NS = 0.1380 (bottom left) and *P = 0.0178 (bottom right). In l, NS = 0.2260 (up), and ****P < 0.0001 (bottom left) and < 0.0001 (bottom right). Statistical significance was determined using a two-tailed, unpaired Student’s t-test.