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. 2000 Jun;66(6):2555–2564. doi: 10.1128/aem.66.6.2555-2564.2000

FIG. 3.

FIG. 3

RAPD fingerprint profiles and Southern blots of Streptomyces, suprageneric, and outgroup DNA primed with 70-34. (a) RAPD fingerprint profiles of Streptomyces spp. Lane 1, DNA mass marker ladder; lane 2, no DNA control; lane 3, Streptomyces lividans 1326; lane 4, Streptomyces antibioticus; lane 5, Streptomyces albus; lane 6, Streptomyces coelicolor; lane 7, Streptomyces rimosus; lane 8, Streptomyces ambofaciens; lane 9, Streptomyces ambofaciens; lane 10, Streptomyces violaceus; lane 11, Streptomyces griseifuscus; lane 12, Streptomyces olivaceus; lane 13, Streptomyces badius 252; lane 14, Streptomyces viridosporus T7A; lane 15, Streptomyces rochei A4; lane 16, Streptomyces lydicus WYEC108; lane 17, Streptomyces hygroscopicus YCED 9; lane 18, Streptomyces hygroscopicus YCED 9; lane 19, Streptomyces plicatus; lane 20, Streptomyces erythraeus. (b) RAPD fingerprint profiles of suprageneric (lanes 3 to 15) and outgroup (lanes 16 to 26) strains. Lane 1, DNA mass marker ladder; lane 2, no DNA control; lane 3, Arthrobacter ramosus; lane 4, Nocardioides simplex; lane 5, Brevibacterium linens; lane 6, Micrococcus luteus; lane 7, Dactylosporangium fulvum; lane 8, Dactylosporangium roseum; lane 9, Corynebacterium xerosis; lane 10, Streptosporangium longisporum; lane 11, Microbispora aerata; lane 12, Frankia sp. strain 33255; lane 13, Gordonia corallina; lane 14, Rhodococcus opacus; lane 15, Thermomonospora mesophila; lane 16, Burkholderia cepacia DLC 62; lane 17, Bacillus subtilis; lane 18, Staphylococcus aureus; lane 19, Flavobacterium spp.; lane 20, Escherichia coli JM101; lane 21, Pseudomonas putida mt-2; lane 22, Ralstonia eutropha JMP134; lane 23, Enterobacter cloacae 96-3; lane 24, Salmonella enterica serovar Typhimurium strain TA 1538; lane 25, Pseudomonas stutzerii KC; lane 26, Clostridium bifermentans KMR-1; lane 27, Streptomyces badius 252, positive control. (Righthand side panels). Southern blot tests of homology with RAPD PCR products. The probe is a gel-purified 0.3-kb RAPD fragment generated from Streptomyces badius 252.