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. 2000 Jun;66(6):2555–2564. doi: 10.1128/aem.66.6.2555-2564.2000

FIG. 4.

FIG. 4

RAPD fingerprint profiles and Southern blots of Streptomyces, suprageneric, and outgroup DNA primed with 70-40. (a) RAPD fingerprint profiles of Streptomyces spp. Lane 1, DNA mass marker ladder; lane 2, no DNA control; lane 3, Streptomyces lividans 1326; lane 4, Streptomyces antibioticus; lane 5, Streptomyces albus; lane 6, Streptomyces coelicolor; lane 7, Streptomyces rimosus; lane 8, Streptomyces ambofaciens; lane 9, Streptomyces ambofaciens; lane 10, Streptomyces violaceus; lane 11, Streptomyces griseifuscus; lane 12, Streptomyces olivaceus; lane 13, Streptomyces badius 252; lane 14, Streptomyces viridosporus T7A; lane 15, Streptomyces plicatus; lane 16, Streptomyces erythraeus; lane 17, Streptomyces rochei A4; lane 18, Streptomyces lydicus WYEC108; lane 19, Streptomyces hygroscopicus YCED 9; lane 20, Streptomyces hygroscopicus YCED 9. (b) Suprageneric (lanes 3 to 13) and outgroup (lanes 19 to 29) strains. Lane 1, DNA mass marker ladder; lane 2, no DNA control; lane 3, Arthrobacter ramosus; lane 4, Brevibacterium linens; lane 5, Micrococcus luteus; lane 6, Dactylosporangium fulvum; lane 7, Corynebacterium xerosis; lane 8, Streptosporangium longisporum; lane 9, Microbispora aerata; lane 10, Frankia sp. strain 33255; lane 11, Gordonia corallina; lane 12, Rhodococcus opacus; lane 13, Thermomonospora mesophila; lane 14, Streptomyces lydicus WYEC108; lane 15, Streptomyces viridosporus T7A; lane 16, Streptomyces rimosus; lane 17, sterile soil sample inoculated with S. lydicus WYEC108; lane 18, unautoclaved soil sample inoculated with S. lydicus WYEC108; lane 19, Burkholderia cepacia DLC 62; lane 20, Pseudomonas stutzerii KC; lane 21, Staphylococcus aureus; lane 22, Flavobacterium spp.; lane 23, Escherichia coli JM101; lane 24, Pseudomonas putida mt-2; lane 25, Ralstonia eutropha JMP134; lane 26, Enterobacter cloacae 96-3; lane 27, Salmonella enterica serovar Typhimurium TA 1538; lane 28, Clostridium bifermentans KMR-1; lane 29, Bacillus subtilis. (Righthand side panels) Southern blot tests of homology with RAPD PCR products. The probe is a gel-purified 0.3-kb RAPD fragment generated from Streptomyces lydicus WYEC108.