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. 2000 Jun;66(6):2555–2564. doi: 10.1128/aem.66.6.2555-2564.2000

FIG. 6.

FIG. 6

(a) RAPD profile of DNA isolated from soil and amplified with primer 70-40. (b) Southern blot probed with the S. lydicus WYEC108 0.3-kb fragment. (a) RAPD fingerprint profile. Lane 1, control field soil; lane 2, control field soil from a different location of the plot; lane 3, bead-mill DNA extraction procedure of 10−2 dilution (initial inoculum concentration, 3 × 109 CFU g−1); lane 4, bead-mill DNA extraction procedure of 10−2 dilution of nonsterilized field soil inoculated with WYEC108 spores; lane 5, DNA extracted by the freeze-thaw method from 10−3 dilution of sterile inoculated soil; lane 6, DNA extracted by the freeze-thaw method from a 10−2 dilution of nonsterile inoculated soil; lane 7, DNA extracted by the freeze-thaw method from a 10−7 dilution of nonsterile inoculated soil; lane 8, WYEC108-positive control template; lane 9, no DNA control; lane 10, DNA mass ladder.