TABLE 3.
DNA isolation comparisonsa
| Strain | Procedure | A260/A280 | DNA concn (μg/μl) |
|---|---|---|---|
| S. badius 252 | Marmur | 0.89 | 0.246 |
| 5′→3′ | 1.20 | 0.717 | |
| Rao | 1.83 | 1.68 | |
| Heath | 1.87 | 2.98 | |
| S. viridosporus T7A | Marmur | 1.12 | 0.342 |
| 5′→3′ | 1.48 | 0.951 | |
| Rao | 1.92 | 5.40 | |
| Heath | 1.84 | 0.500 | |
| Streptomyces sp. strain WYE 27 | Marmur | 1.28 | 1.077 |
| 5′→3′ | 1.15 | 0.426 | |
| Rao | 1.90 | 4.14 | |
| Heath | 1.54 | 3.71 | |
| Streptomyces sp. strain YCED 5 | Marmur | 1.09 | 0.291 |
| 5′→3′ | 1.01 | 0.327 | |
| Heath | 1.90 | 0.97 |
a
Cell pellets were normalized on all small-scale DNA-extracted samples to 0.2-ml centrifuged pellet volume.