Table 2.

Number of amino acid mutations in different gene regions

Gene		Groupa	Day 22	Day 29	Day 36	Day 62	Day 75	Day 78	Day 83	Day 86	cf BA.5.2.1 (Outbreak.info)
ORF1a		Major	9	9	9	10	10	10	10	11	8
		Minor	9	4	21	11	10	9	14	7	0
ORF1b	nsp12 is encoded	Major	4	5	5	7	9	9	7	9	4
		Minor	5	3	29	11	3	3	8	3	0
ORF2	Spike protein	Major	31	31	31	35	38	38	37	39	31
		Minor	0	1	7	13	3	7	10	6	0
ORF3a		Major	1	1	1	1	1	1	1	1	1
		Minor	0	1	2	0	0	0	1	0	0
ORF4	Envelope protein	Major	1	1	1	1	1	1	1	1	1
		Minor	0	0	0	0	0	0	0	0	0
ORF5	Membrane protein	Major	3	3	3	3	3	3	3	3	3
		Minor	2	0	2	0	0	0	0	0	0
ORF6		Major	0	0	0	0	0	0	0	0	0
		Minor	2	3	2	0	0	2	1	0	0
ORF7a/b		Major	0	0	0	0	0	0	0	0	0
		Minor	0	1	0	0	0	0	0	0	0
ORF8		Major	0	0	0	0	1	1	1	0	0
		Minor	0	0	0	0	0	0	1	0	0
ORF9	Nucleocapsid protein	Major	5	5	5	5	5	5	5	6	4
		Minor	1	1	1	3	2	3	2	2	0
ORF10		Major	0	0	0	0	0	0	0	0	0
		Minor	0	0	0	0	0	0	0	0	0
All		Major	54	55	55	62	68	68	65	70	52
		Minor	19	14	64	38	18	24	37	18	0
		Total	73	69	119	100	86	92	102	88	52

^aThe major and minor mutations in this table are defined by a frequency of more and less than 50%, respectively.