Figure 1.

The amplification curves (top) and the standard curve (bottom) of the single and multiplex real-time PCR assay. (A–C) The amplification curves (top) and the standard curve (bottom) of single real-time PCR assay for detection of PEDV (A), PoRV (B), and PDCoV (C), respectively, with concentrations of each plasmid standard ranging from 1.0 × 10⁸ to 1.0 × 10¹ copies/μL. (D) Amplification curves (top) and standard curves (bottom) of optimized multiplex real-time PCR for simultaneous detection of PEDV, PoRV, and PDCoV. The concentrations of each plasmid standard are from 1.0 × 10⁸ to 1.0 × 10³ copies/μL.