Table 3.

The repeatability tests of multiplex real-time PCR.

Templates	Concentrations (copies/μL)	Intra-assay		Inter-assay
Cq values (mean ±SD)	CV%	Cq values (mean ±SD)	CV%
PEDV	1 × 108	11.53 ± 0.05	0.40	11.41 ± 0.16	1.38
	1 × 107	15.45 ± 0.09	0.55	15.30 ± 0.15	0.98
	1 × 106	19.00 ± 0.13	0.68	18.84 ± 0.18	0.96
	1 × 105	22.19 ± 0.05	0.21	21.93 ± 0.17	0.78
	1 × 104	25.75 ± 0.11	0.43	25.31 ± 0.14	0.56
	1 × 103	29.02 ± 0.15	0.50	28.61 ± 0.11	0.38
	Negative control	ND	ND	ND	ND
PoRV	1 × 108	12.96 ± 0.11	0.85	12.89 ± 0.21	1.63
	1 × 107	16.77 ± 0.15	0.87	16.93 ± 0.23	1.33
	1 × 106	20.03 ± 0.06	0.29	20.16 ± 0.18	0.88
	1 × 105	23.18 ± 0.03	0.15	23.23 ± 0.16	0.68
	1 × 104	26.66 ± 0.08	0.11	26.59 ± 0.35	1.33
	1 × 103	29.53 ± 0.11	0.39	29.58 ± 0.16	0.52
	Negative control	ND	ND	ND	ND
PDCoV	1 × 108	13.36 ± 0.11	0.83	13.73 ± 0.02	0.11
	1 × 107	17.39 ± 0.14	0.80	17.47 ± 0.04	0.22
	1 × 106	21.03 ± 0.03	0.14	21.10 ± 0.03	0.14
	1 × 105	24.15 ± 0.10	0.42	24.20 ± 0.06	0.25
	1 × 104	27.72 ± 0.16	0.56	27.61 ± 0.06	0.23
	1 × 103	30.37 ± 0.04	0.12	30.58 ± 0.23	0.76
	Negative control	ND	ND	ND	ND

ND means no data.