FIG. 3.

(A) Survival of L. salivarius UCC 118 during spray drying in 20% (wt/vol) RSM supplemented with 0.5% (wt/vol) yeast extract and 1% (wt/vol) sucrose at different air outlet temperatures (bar graph). The line indicates the moisture contents of the resulting powders. The air inlet temperature was maintained at 170°C. The results are means based on data from duplicate spray-drying trials, and standard deviations are indicated by vertical bars. (B and C) CSLM micrographs of UCC 118-containing powders produced at air outlet temperatures of 60 to 65°C (B) and 90 to 95°C (C). The powders were stained with the LIVE/DEAD BacLight viability stain; live cells are green, and dead cells are red. Bars = 10 μm.