Figure 4.

Small variations in the anesthesia level determine substantial changes in the late component of the sensory-evoked response

(A) Allen Mouse Brain Atlas parcellation used to identify the cortical regions for single-trial analysis (M1, primary motor cortex; M2, Secondary motor cortex; SSbdf, primary somatosensory barrel field cortex; SStr, primary somatosensory cortex trunk; RSP, Retrosplenial cortex; V1, primary visual cortex; L, left; R, right).

(B) Sensory-evoked calcium activity in the Barrel field cortex under deep (on the right) and medium (on the left) anesthesia in the same representative animal (dashed line indicates the timing of the stimulus, n stimuli = 11). Column chart showing onset time (C), time to peak (D), FWHM (E), ΔF/F% (F), rise slope (G) and decay slope (H) of the early component for six cortical regions in the left (L) and in the right (R) hemisphere under deep (patterned columns) and medium (empty columns) anesthesia. Whisker length is the standard error.

(I) Boxplot showing the probability of late response for six cortical regions in the left (L) and in the right (R) hemisphere under (patterned columns) and medium (empty columns) anesthesia. Whisker length is the standard error. Boxplot showing comparing time to peak (J), ΔF/F% (k) and FWHM (L) of the early (empty columns) and late component (patterned columns) for six cortical regions in the left (L) and right (R) hemisphere under medium anesthesia. N mice = 6. two-way ANOVA with Bonferroni correction (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001), Data are shown as mean ± SEM.