Fig. 10. Triazole stress does not reduce Hmg1 protein abundance.

A Localization analyses of Hmg1-GFP in response to sub-lethal triazole stress in the hmg1^WT-gfp and hmg1^S305P-gfp strains. All images were captured with an exposure time of 600 ms. Note apparent increase in intensity and mislocalization of the GFP fluorescent signal at 0.0625 µg/ml voriconazole. Scale bar = 50 µm. Microscopy experiments were completed three times independently with similar results. B Quantitation of GFP in lysates of the hmg1^WT-gfp and hmg1^S305P-gfp strains cultured for 12 hrs in the presence of 0, 0.03125, or 0.0625 µg/ml voriconazole. Data are presented as the mean ± standard deviation. Assays were performed in biological triplicate and statistical analyses completed using one-way ANOVA and Tukey’s Test post-hoc. Despite a trend for increased GFP abundance in the presence of voriconazole, no significant differences were noted among the groups. Source data are provided as a Source Data file.