TABLE 1.
Effect of shaking parameters on OTRs in square deep-well microtiter plates
| Type of movement | Angle with horizontal plane | Rotating velocity (rpm) | Diam (cm) | Froude no.a | OTRs (mmol of O2 liter−1 h−1)b at 3 different aqueous vols (μl)
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| 500
|
750
|
1,000
|
||||||||
| Biol.c | Chem.d | Biol. | Chem. | Biol. | Chem. | |||||
| None | 0° | 0 | 0 | 2.1 | NDe | 1.6 | ND | 1.6 | ND | |
| Orbital shaking | 0° | 300 | 2.5 | 1.3 | 12.5 | 11.0 ± 0.8 | 7.4 ± 0.9 | 7.5 ± 0.2 | 3.5 | 5.8 ± 0.4 |
| 30° | 300 | 2.5 | 1.3 | 22.0 ± 0.6 | ND | 12.0 ± 0.6 | ND | 10.2 | ND | |
| 0° | 375 | 2.5 | 2.0 | 19.4 | ND | 15.1 | ND | 10.9 | ND | |
| 0° | 300 | 5 | 2.5 | 37.8 ± 2.6 | 27.3 ± 0.4 | 23.8 ± 2.2 | 19.8 ± 0.4 | 17.7 ± 0.7 | 16.3 ± 0.1 | |
| Rotation | 75° | 60 | 18 | 0.7 | 20.7 | ND | 15.7 | ND | 12.5 | ND |
a
The Froude number represents the ratio between centrifugal and gravitational acceleration.
b
Values are averages of three or more measurements ± standard deviations. Single values are results of single measurements.
c
Biol. indicates OTRs determined from growth curve of P. putida CA-3 on a glucose mineral medium.
d
Chem. indicates OTRs determined by the sulfite oxidation method.
e
ND, Not determined.