TABLE 2.
Replication from frozen master cultures and subsequent growth of various strains
| Organism | No. of strains (code[s]a) | Avg no. of CFU replicated (103)b | Avg OD540
|
|
|---|---|---|---|---|
| After inoculationc | After 3 days of shakingd | |||
| Achromobacter xylosoxidans | 1 (h6) | 10 | 0.07 | 20 |
| Alcaligenes sp. | 3 (d12, e1, g3) | 9 | 0.08 ± 0.02 | 0.20 ± 0.13 |
| Pseudomonas azotoformans | 2 (d4, g5) | 35 | 0.14 | 29 |
| Pseudomonas cepacia | 1 (h2) | 50 | 0.19 | 46 |
| Pseudomonas fluorescens | 2 (g1, h12) | 35 | 0.13 | 17 |
| Pseudomonas fulva | 2 (d8, f6) | 20 | 0.06 | 32 |
| Pseudomonas marginalis | 6 (c9, c11, e3, e5, f8, f10) | 50 | 0.07 ± 0.06 | 21 ± 10 |
| Pseudomonas mendocina | 1 (g11) | 20 | 0.05 | 0.26 |
| Pseudomonas putida | 5 (a9, g9, h4, h8, h10) | 4 | 0.12 ± 0.07 | 26 ± 8 |
| Pseudomonas stutzeri | 3 (d6, f4, g7) | 26 | 0.13 ± 0.03 | 32 ± 9 |
| Pseudomonas syringae | 6 (b10, c1, c7, d10, f2, f12) | 39 | 0.14 ± 0.06 | 30 ± 7 |
| Pseudomonas viridiflava | 1 (b12) | 5 | 0.06 | 34 |
| Rhodococcus erythropolis | 2 (a3, a5) | 58 | 0.20 ± 0.04 | 12 |
| Rhodococcus globerulus | 5 (a1, a11, b4, e7, e11) | 8 | 0.13 ± 0.05 | 9.6 ± 1.3 |
| Rhodococcus opacus | 6 (a7, b2, b6, b8, c5, d2) | 3 | 0.01 ± 0.01 | 16 ± 11 |
| Rhodococcus sp. | 1 (e9) | 1 | 0.09 | 38 |
| Variovorax sp. | 1 (c3) | 50 | 0.12 | 0.16 |
a
Strain codes from the authors' laboratory (corresponding to their position on the master plate).
b
Number of CFU transferred from the frozen master cultures using the spring-loaded replicator, as measured by the number of colonies on nutrient broth agar.
c
OD540 of 750-μl cultures just after inoculation with colonies from a nutrient broth agar plate.
d
OD540 of 750-μl cultures (mineral medium, 150 mM glucose, 10 mM aspartate) after 3 days of incubation at 25°C (300 rpm; shaking diameter, 5 cm).