REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Fresh resections of tumor tissue and corresponding benig tissue from PCa patients	Heidegger et al., Molecular Cancer 2022	https://doi.org/10.1186/s12943-022-01597-7
Fresh resections of tumor tissue and corresponding benig tissue from NSCLC patients	This paper.	N/A
Critical commercial assays and reagents
BD TuDoR™ dissociation reagent	BD Biosciences	Cat#: 661563
BD Pharm Lyse™	BD Biosciences	Cat#: 555899
BD Rhapsody™ Cartridge Kit	BD Biosciences	Cat#: 633733
BD Rhapsody™ Cartridge Reagent Kit	BD Biosciences	Cat#: 633731
BD™ Human Single-Cell Multiplexing Kit	BD Biosciences	Cat#: 633781
BD Rhapsody™ WTA Amplification Kit	BD Biosciences	Cat#: 633801
BD Rhapsody™ cDNA Kit	BD Biosciences	Cat#: 633773
AMPure XP	Beckman Coulter	Cat#: A63880
Qubit™ dsDNA HS Assay Kit	Invitrogen	Cat#: Q32854
High Sensitivity D1000 Reagents	Agilent	Cat#: 5067-5585
High Sensitivity D5000 Reagents	Agilent	Cat#: 5067-5593
High Sensitivity D1000 ScreenTape	Agilent	Cat#: 5067-5584
High Sensitivity D5000 ScreenTape	Agilent	Cat#: 5067-5588
High Sensitivity RNA ScreenTape	Agilent	Cat#: 5067-5579
High Sensitivity RNA ScreenTape Sample Buffer	Agilent	Cat#: 5067-5580
High Sensitivity RNA ScreenTape Ladder	Agilent	Cat#: 5067-5581
RNeasy Mini Kit	Qiagen	Cat#: 74104
BD Pharmingen™ 7-AAD	BD Biosciences	Cat#: 559925
Calcein AM	Invitrogen	Cat#: C1430
Draq7	BD Biosciences	Cat#: 564904
ultraView Universal DAB Detection Kit	Roche	Cat#: 5269806001
Antibodies used for immunohistochemistry
CD45 (clone 2B11&PD7/26)	Roche	Cat#: 5269423001
P504s (clone SP116)	Roche	Cat#: 8035130001
p63 (clone 4A4)	Roche	Cat#: 5867061001
Deposited data
BD Rhapsody dataset (in H5AD format)	This study	https://zenodo.org/record/8063560
10X Chromium dataset	Heidegger et al., Molecular Cancer 2022	GSE193337
Software and algorithms
Seven Bridges - BD Rhapsody™ WTA Analysis Pipeline	Seven Bridges Genomics	v1.7.1
Cellranger v5.0.0	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger
QuPath	Bankhead et al., Scientific Reports 2017	https://qupath.github.io
M3Drop	Andrews et al., Bioinformatics 2019	https://github.com/tallulandrews/M3Drop
Integrative Genomics Viewer (IGV)	Robinson JT et al., Nature Biotechnology 2011	https://software.broadinstitute.org/software/igv/
GraphPad Prism	Graphpad	v9
scanpy	Wolf et al., Genome Biology 2018	https://github.com/scverse/scanpy
scVI	Gayoso et al., Nature Biotechnology 2022	https://github.com/scverse/scvi-tools
decoupler-py	Badia-i-Mompel P et al., Bioinformatics Advances 2022	https://github.com/saezlab/decoupler-py
DESeq2	Love et al., Genome Biology 2014	https://github.com/thelovelab/DESeq2