Figure 2.

Intracellular symbiont localization in the symbiotic organ of Notosacantha leaf beetles

FISH visualization of the nucleated and symbiont-free basal region and the symbiont-harboring apical region of the epithelial cells of the gut-associated symbiotic organ of Notosacantha ihai (A) and Notosacantha nishiyamai (B). Magenta, blue, and green signals show FISH signals targeting symbiont 16S rRNA, DAPI signals targeting host nuclear DNA, and fluorescent phalloidin signals targeting muscular actin filaments, respectively. n, nucleus; s, symbiont.