Fig. 1. Particle size, morphology, and stability of linear-comb PLA-POEGMA copolymer nanoparticles compared to linear–linear PLA-PEG nanoparticles: (A–C) transmission electron microscopy images of nanoparticles fabricated using (A) P(L)LA10K-b-PEG10K; (B) P(L)LA10K-b-POEGMA47510K; and (C) P(L)LA10K-b-POEGMA200010K (scale bars = 200 nm); (D and E) DLS stability data for linear and comb block copolymer-based nanoparticles fabricated using (D) THF or (E) DMF as a solvent via flash nanoprecipitation over a 15 day incubation time in PBS at 37 °C (n = 3, data points represent the mean values, error bars represent the standard deviation). Beyond day 3 in DMF (panel E, grey arrow) P(L)LA10k-b-POEGMA200010k exhibited large sizes and polydispersity values indicative of nanoparticle aggregation.
