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. 2024 Apr 30;23:124. doi: 10.1186/s12934-024-02392-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Rational design of Mfr promoters. (A) Sequences of tested promoters with key features highlighted in bold. (B) Gene transcription analysis of Nluc reporter gene cloned downstream of promoters A0, A1mut, A1prib and A2 at exponential phase (20 h). Fold change (Fc) expressed in relation to housekeeping genes rpsM, gapdh and gyrA calculating ΔΔCt (see Methods). This experiment was performed twice with three technical replicates (N = 2). Statistical test was performed using One-way ANOVA with multiple comparisons. (C) Nluc production measured by luminescence at different time points normalised by total protein concentration determined by BCA. This experiment was performed twice with two technical replicates (N = 2). P-value: (*, < 0.0332; **, < 0.0021; ***, < 0.0002; ****, < 0.0001). Data is shown as average ± standard deviation (SD)