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. 2024 Apr 30;23:124. doi: 10.1186/s12934-024-02392-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Secretion signal analysis. (A) Secretion efficiency of selected signal peptides. Mfr strains expressing several signal peptides with a luminescent reporter were grown to exponential phase. Luminescence of both supernatant (extracellular) and pellet (intracellular) was measured to obtain a secretion efficiency ratio. Data are shown as average +/- SD of two biological and technical replicates (N = 2). Statistical analysis was performed using One-way ANOVA test of multiple comparisons. The statistical analysis shown both compared to s3040 (*) and s515 (#) (p-value < 0.0002). (B) Luminescence signal of the supernatant in s3040 and s515. The signal was measured and normalised by quantifying the total protein in the pellet. Data are shown as average +/- SD of two biological and technical replicates (N = 2). Statistical analysis was performed using One-way ANOVA test of multiple comparisons (*, p-value < 0.05)