Table 1.

Mfr secretion signals identified by SignalP 6.0 server. All Mfr ORFs were evaluated. Signal sequences are shown divided by their three characteristic regions: the positively charged, the hydrophobic transmembrane and the polar at the C-terminal. The added residues after the post-cleavage sites also appear. Tested sequences are in bold. Lipobox motifs appear underlined. Membrane anchor means that there is a predicted C-terminal membrane helix that could anchor the protein after cleavage of the signal peptide. Suppl. File 2 summarises the AUC for the secreted proteins

# ID	SP (Sec/SPI)	Signal Peptide			Post-cleavage added sequence	Secretome (MS)	Membrane Anchor/Lipobox
		Positive charge	Hydrophobic transmembrane	C-terminal
s55	0.85	MKK	LLTILTTLIGT	SGSISA	VVSCKGG	Yes	Lipobox
s515	0.82	MK	ITAILSSLFLSPTL	LNT	SPILVNGG	No	Membrane Anchor
s1260	0.58	MKK	LLSILAICTLATTSILLSPLL	INNNSNNNIVLKA	ETKKEGG	No	Signal Peptide
s1535	0.54	MNLLKKKKNK	ILAFAILAGLMTSASLGSTVFYSIA	ADNSLA	KDVDSGG	No	Membrane anchor
s1545	0.66	MNLLKKKKNK	ILAFAILAGLMTSASLGSTVFYSI	ADNSLA	KEVDSGG	Yes	Membrane anchor
s1575	0.53	MKINKNHSR	LLKLISIVTITSSSIILPSF	LVT	KNQESGG	No	Membrane anchor
s2165	0.99	MKK	LLTLLTISTLLVI	PTSSS	FLINKGG	No	Signal Peptide
s3040	0.99	MK	LFLPTLFLLSNSITP	SLA	NSVNVVNGG	No	Membrane Anchor
s3290	0.5	MKLVKK	LGFLSLSAISILGPL	AA	INNLTDNNGG	Yes	Membrane Anchor
s3620	0.61	MKK	VLGITLLGSII	ATASA	SVVSCSVGISLDGG	No	Lipobox
s3960	0.48	MKK	LLALLAVTSILT	SSGITY	VIHENGG	Yes	Signal Peptide
s3965	0.78	MKK	LLAVLIGLTLFTT	SGVSYV	AYDNIGG	Yes	Signal Peptide