TABLE 3.

Detection of P. savastanoi pv. savastanoi in asymptomatic stem tissues from infected olive plants

Variety	No. of samples analyzed	P. savastanoi pv. savastanoi detection (no. of positive samples)a
		Without preenrichment		With preenrichment
				King's B		PVF-1
		Bacterial isolation	PCR and restriction analysis	Bacterial isolation	PCR and restriction analysis	Bacterial isolation	PCR and restriction analysis
Blanqueta	6	1	0	0	1	0	3
Gordal	3	2	0	0	3	1	2
Manzanilla	6	2	0	3	4	0	5
Picual	20	5	4b	4	6	4	10
Picudo	3	0	0	1	2	1	3
Total no. of samples	38	10	4	8	16	6	23

^aThat is, the number of samples in which P. savastanoi pv. savastanoi was detected. Bacterial isolations were performed by plating serial dilutions on King's B and PVF-1 media, and after bacterial purifications, isolates were identified as described in Materials and Methods. PCR amplification was done by using the primer pair IAALF-IAALR, and subsequently, the amplified product was digested with the restriction enzyme HaeIII as described in Materials and Methods. 

^bOne of these amplified fragments was double strand sequenced, thus verifying iaaL detection.