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. Author manuscript; available in PMC: 2024 Sep 22.
Published in final edited form as: Science. 2024 Mar 21;383(6689):1344–1349. doi: 10.1126/science.adj3566

Fig. 1. 760 kb HAC constructs efficiently acquire centromeres and exist as autonomous chromosomes.

Fig. 1.

(A) Schematic of approach to generate a HAC. (B) Representative images of a single-copy HAC generated in HT1080 and U2OS cells. Insets: 5x magnification. Bar, 5 μm. See also Table S1. (C) Quantification of proportion of “small HACs” (FISH signal spans less than 1 μm), “large HACs” (FISH signal spans greater than 1 μm), “integrations” and “no signal” spreads generated from HAC formation assays. The mean (+/− SD) is shown. (D) Comparison of size of a HAC made from YAC-Mm-4q21LacO and a multimerized HAC made from 4q21 BACLacO. Both HACs are shown at the same scale. Coloring is identical to what is shown in the merged images in panel B. Bar, 1 μm.