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. Author manuscript; available in PMC: 2024 Sep 22.
Published in final edited form as: Science. 2024 Mar 21;383(6689):1344–1349. doi: 10.1126/science.adj3566

Fig. 3. YAC-Mm-4q21LacO HACs are functional as single copy DNA.

Fig. 3.

(A) Schematic of approach used to enrich single copy HACs. (B) Representative image of HACs isolated by sucrose gradient with either a single or two foci of LacO. The proportion of HACs with a single or two foci is noted. HACs with two LacO foci also had two CENP-A foci suggesting that they are mitotic. Bar, 1 μm. (C) Representative image of a multimerized HAC (Clone 27 from (16)) from mitotic chromosome spreads. Bar, 1 μm.